Review the PCR protocol herein and identify its name. A single- stranded probe with internal complementary sequences folds on itself into a "hairpin" shape bringing a fluorescent molecule at one end of the strand in proximity to the quencher molecule at the other end of the strand. After PCR product is formed, during the next denaturation step the probe becomes single- stranded, binds to PCR target DNA, and separates the fluorescent molecule from the quencher molecule. PCR product is detected as increased fluorescence.
A) Molecular beacon PCR
B) 5' RACE PCR
C) Reverse transcriptase PCR
D) Branched chain PCR
E) TaqMan PCR
Correct Answer:
Verified
Q7: Why is intact mRNA so hard to
Q8: Which detection method below is frequently used
Q9: Reverse transcriptase was discovered in
A)retroviruses.
B)adenoviruses.
C)herpes viruses.
D)Coxsackie viruses.
E)polioviruses.
Q10: Which is more sensitive, nucleic acid amplification
Q11: What advantage does DNA polymerase from Thermus
Q13: When making mRNA preparations for reverse transcriptase
Q14: The three basic steps of polymerase chain
Q15: Which of the following is true regarding
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