How does chemical degradation DNA sequencing (Maxam- Gilbert) differ from the dideoxy chain termination (Sanger) method?
A) Agarose gel is used for electrophoresis instead of high resolution polyacrylamide gel.
B) Ethidium bromide is used to visualize the DNA fragments instead of radioactive sulfur or phosphorus.
C) A lower ratio of dideoxy:deoxy nucleotides is used.
D) "Rare cutter" restriction enzymes or nucleotide chemical modification are used to localize each base.
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