When staining cells for flow cytometry, which technique would have the least amount of nonspecific staining?

Question

Quizplus · Accepted Answer

Monoclonal antibodies are designed to target a single specific epitope, reducing the likelihood of cross-reactivity and nonspecific binding compared to polyclonal antibodies. Direct staining, where the antibody is directly conjugated to a fluorophore, eliminates the need for a secondary antibody, further reducing the potential for nonspecific staining.

When Staining Cells for Flow Cytometry, Which Technique Would Have