A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?
A) Use electrophoresis to identify plasmids containing an insert of the expected size.
B) Use a labeled synthetic probe complementary to the gene sequence.
C) Sequence the DNA of the plasmids from each isolate.
D) Use a microarray to detect a transcript of the gene.
E) Assay for activity of the gene product.

Question

Quizplus · Accepted Answer

Using a labeled synthetic probe complementary to the gene sequence allows for specific identification of clones containing the gene of interest by hybridization, making it an efficient method.

A Researcher Inserted DNA Fragments from an Organism into Plasmids