What is one major limitation of two -dimensional gel electrophoresis (2DGE)?
A) When products are separated, they tend to leach out of the gel matrix.
B) Only the most abundant products are detected.
C) 2DGE can be run only on nucleic acids.
D) It is extremely costly to execute in a typical molecular biology laboratory.
E) 2DGE is useful only in separating eukaryotic gene products.

Question

Quizplus · Accepted Answer

2DGE is limited in detecting only the most abundant proteins, as less abundant proteins may not be visible due to their low concentration.

What Is One Major Limitation of Two -Dimensional Gel Electrophoresis