In 1964, Nirenberg and Leder used the triplet binding assay to determine specific codon assignments. A complex of which of the following components was trapped in the nitrocellulose filter?
A) uncharged tRNAs and ribosomes
B) sense and antisense strands of DNA
C) free tRNAs
D) charged tRNA, RNA triplet, and ribosome
E) ribosomes and DNA

Question

Quizplus · Accepted Answer

The triplet binding assay involved charged tRNA, an RNA triplet, and a ribosome forming a complex that was trapped on the nitrocellulose filter, allowing the determination of specific codon assignments.

In 1964, Nirenberg and Leder Used the Triplet Binding Assay