What is the medium used in the membrane filter (MF) procedure to differentiate fecal coliforms and Escherichia coli?

Question

Quizplus · Accepted Answer

Eosin-methylene blue (EMB) agar is a differential medium commonly used in the membrane filter procedure to differentiate fecal coliforms and E. coli. EMB agar contains dyes that inhibit the growth of gram-positive bacteria and allow for the detection of lactose fermentation. E. coli, which is a fecal coliform, will produce metallic green colonies on EMB agar due to its ability to ferment lactose and reduce the dyes in the medium. Other fecal coliforms will produce pink colonies on EMB agar. Thus, EMB agar is the best choice for differentiating fecal coliforms and E. coli in the MF procedure.

What Is the Medium Used in the Membrane Filter (MF)