The blotting of proteins from polyacrylamide gels to nitrocellulose paper, and the subsequent identification by specific antibodies, is called a(n) _________blot.

Question

Quizplus · Accepted Answer

The description given in the question matches the Western blotting technique, which involves transferring proteins from a gel matrix to a nitrocellulose or PVDF membrane and detecting the specific proteins using antibodies. Southern blotting is used for detecting specific DNA sequences, Northern blotting is used for detecting specific RNA sequences, and Eastern blotting is a less commonly used technique for detecting post-translational modifications of proteins.

The Blotting of Proteins from Polyacrylamide Gels to Nitrocellulose Paper