DGGE and T- RFLP are both powerful molecular microbial ecology methods that use multiple genes to look at diversity.

Question

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Both DGGE (Denaturing Gradient Gel Electrophoresis) and T-RFLP (Terminal Restriction Fragment Length Polymorphism) use multiple genes to study microbial diversity. DGGE separates DNA fragments based on their sequence and melting behavior, while T-RFLP uses restriction enzymes to cut DNA at specific sites and then separates the resulting fragments based on their size.

DGGE and T- RFLP Are Both Powerful Molecular Microbial Ecology