In the acid- fast (Ziehl- Neelsen) stain, the role of the phenol is to enhance penetration of the crystal violet into the cell wall.

Name: In the acid- fast (Ziehl- Neelsen) stain, the role of the phenol is to enhance penetration of the crystal violet into the cell wall.
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The role of phenol is to act as a decolorizing agent in the acid-fast stain, which removes the crystal violet from non-acid-fast cells, allowing for the detection of acid-fast cells (which retain the stain).

In the Acid- Fast (Ziehl- Neelsen) Stain, the Role of the Phenol