You have performed the following mating experiment using Hfr and F- strains of Escherichia coli: Hfr (thr+ leu+ gal+ strs) × F- (thr- leu- gal- strr) . Which of the following selective media would you use to score recombinant colonies?
A) minimal medium + streptomycin
B) minimal medium + appropriate nutrient
C) minimal medium + streptomycin + appropriate nutrient
D) minimal medium
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