During dideoxy sequencing reactions,dideoxyribonucleoside triphosphates ddNTPsare added in small amounts to terminate the sequencing reaction.How do ddNTPs stop a sequencing reaction?
A)ddNTPs lack a 5′ phosphate group.
B)ddNTPs have a hydroxyl group at their 5′ end.
C)ddNTPs are irreversible inhibitors on polymerases.
D)ddNTPs lack a hydroxyl -OHgroup at their 3′ end.

Question

Quizplus · Accepted Answer

ddNTPs lack a hydroxyl - OH )
 group at their 3 ′ end .

During Dideoxy Sequencing Reactions,dideoxyribonucleoside Triphosphates DdNTPsare Added in Small Amounts