Why is phage lambda-Red ssDNA-binding protein beta used in MAGE (Multiplex Automated Genome Engineering)?
A) to facilitate phage packaging
B) to inhibit supercoiling
C) to promote supercoiling
D) to promote ligation
E) to promote annealing

Question

Quizplus · Accepted Answer

Phage lambda-Red ssDNA-binding protein beta is used in MAGE to promote annealing. This is because MAGE relies on the annealing of short synthetic oligos to genomic DNA in order to introduce desired changes. The binding protein helps to stabilize the annealing process and increase the efficiency of the desired modifications.

Why Is Phage Lambda-Red SsDNA-Binding Protein Beta Used in MAGE