In the yeast two-hybrid system,the Gal4 DNA binding domain is unable to activate transcription when it is bound to a promoter.

Question

Quizplus · Accepted Answer

The Gal4 DNA binding domain does not have the ability to activate transcription on its own, and requires the Gal4 activation domain to stimulate transcription. Therefore, when it is bound to a promoter, it cannot activate transcription without the activation domain.

In the Yeast Two-Hybrid System,the Gal4 DNA Binding Domain Is