Which of the following is required in ChIP-on-chip but not when using a microarray to analyze transcript abundance?

Question

Quizplus · Accepted Answer

ChIP-on-chip involves the use of an antibody to isolate DNA fragments bound by a specific protein, while microarrays for transcript analysis rely on hybridization of fluorescently-labeled cDNA to probe sequences on a microarray. PCR may or may not be used in both techniques, depending on the specific protocol. A microarray is only required for transcript abundance analysis, not for ChIP-on-chip.

Which of the Following Is Required in ChIP-On-Chip but Not