The output of pyrosequencing would be most different from the output of Sanger sequencing for which of the following sequences?
A) CATATG
B) ATGATG
C) CGCGCG
D) TTTTTT
E) CACACA

Question

Quizplus · Accepted Answer

Pyrosequencing struggles with homopolymeric regions (sequences with long stretches of the same nucleotide) like "TTTTTT" in option D, leading to inaccuracies in the output. This is because pyrosequencing measures the release of pyrophosphate upon nucleotide incorporation, and in homopolymeric regions, it's challenging to accurately quantify the number of nucleotides added. Sanger sequencing, on the other hand, does not have this limitation and can sequence homopolymeric regions more accurately.

The Output of Pyrosequencing Would Be Most Different from the Output