What technique would you use to best determine when and in what cells of a given tissue a specific gene is expressed?

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Quizplus · Accepted Answer

In situ hybridization allows for the visualization of where a specific gene is expressed within a tissue sample. This technique involves using a labeled probe that recognizes the RNA transcript of a specific gene and can be visualized under a microscope. It allows for the identification of specific cells that are expressing the gene of interest within a tissue sample. Northern analysis and RT-PCR can also be used to detect the expression of a specific gene, but they do not provide information on the location of the expression within the tissue. Southern analysis and PCR are not suitable for detecting gene expression, as they are used to detect DNA sequences rather than RNA transcripts.

What Technique Would You Use to Best Determine When and in What