Mutations altering the amount of protein synthesized from a reporter construct occur most often in which of the following sequences of DNA?
A) the reporter gene
B) cis-acting DNA elements
C) X-gal coding sequence
D) RNA polymerase II gene
E) the introns of the reporter gene

Question

Quizplus · Accepted Answer

Mutations in cis-acting DNA elements, such as enhancers or promoters, can affect the amount of protein synthesized from a reporter construct by altering the level of expression of the reporter gene. Mutations in the other sequences listed may affect other aspects of gene expression or translation, but are less likely to directly impact the amount of protein synthesized.

Mutations Altering the Amount of Protein Synthesized from a Reporter