In a two-factor ANOVA the main effects of factors A and B should be tested before testing for the presence of the interaction.

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In a two-factor ANOVA, the interaction between factors A and B should be tested first because if there is a significant interaction, it implies that the effect of one factor depends on the level of the other factor, which can affect the interpretation of the main effects.

In a Two-Factor ANOVA the Main Effects of Factors a and B