Engelmann's investigation to determine the effective wavelengths of light in photosynthesis depended on a photosynthetic alga's A)use of CO₂. B)release of O₂. C)use of H₂O. D)use of ATP. E)production of carbohydrate.

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Engelmann's experiment used the release of oxygen by photosynthetic algae to identify the wavelengths of light that were most effective for photosynthesis. He observed the movement of oxygen-seeking bacteria towards areas where the algae were exposed to specific wavelengths of light, indicating oxygen production.

Engelmann's Investigation to Determine the Effective Wavelengths of Light in Photosynthesis