In the ONPG and MUG test for fecal coliforms, a sample that is positive for E. coli will be
A) positive for ONPG; non-fluorescent in UV light.
B) positive for ONPG; fluorescent in UV light.
C) negative for ONPG; fluorescent in UV light.
D) negative for ONPG; non-fluorescent in UV light.

Question

Quizplus · Accepted Answer

E. coli is positive for ONPG, indicating it can hydrolyze ONPG to produce a yellow color, and it is fluorescent under UV light due to the presence of the enzyme β-glucuronidase, which hydrolyzes MUG.

In the ONPG and MUG Test for Fecal Coliforms, a Sample