The following steps must be performed to make a bacterium produce human protein X: 1- Translation; 2- Restriction enzyme; 3- Prokaryotic transcription; 4- DNA ligase;
5- Transformation; 6- Eukaryotic transcription; 7- Reverse transcription. Put the steps in the correct sequence.

Question

Quizplus · Accepted Answer

To produce a human protein in a bacterium, the process starts with eukaryotic transcription (6) of the human gene, followed by reverse transcription (7) to create cDNA from the mRNA. This cDNA is then cut with a restriction enzyme (2) and joined into a bacterial plasmid using DNA ligase (4). The plasmid is introduced into the bacterium by transformation (5), followed by prokaryotic transcription (3) of the inserted human gene and finally translation (1) to produce the human protein.

The Following Steps Must Be Performed to Make a Bacterium