The following events occur if human cells expressing HLA- I are mixed with anti- HLA- I, complement, and trypan blue. What step indicates the cells are HLA- I?

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Quizplus · Accepted Answer

Trypan blue is a dye that enters cells with compromised membranes. If the cells are expressing HLA-I and are mixed with anti-HLA-I antibodies and complement, the complement system is activated, leading to the formation of the membrane attack complex (MAC), which creates pores in the cell membrane. This allows trypan blue to enter the cells, indicating they are HLA-I positive.

The Following Events Occur If Human Cells Expressing HLA- I