Put the following steps of the production of a recombinant vector, containing a human gene, in order:
I. Introduce the recombinant plasmid into a bacterial cell.
II. Isolate the human gene and the vector DNA using restriction enzymes.
III. Ligate the DNA fragments to produce a recombinant plasmid.
IV. Grow bacterial cells on a medium containing a selective agent (such as an antibiotic).

Question

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First, the human gene and vector DNA are isolated using restriction enzymes (II). Then, the DNA fragments are ligated to produce a recombinant plasmid (III). Next, the recombinant plasmid is introduced into a bacterial cell (I). Finally, bacterial cells are grown on a medium containing a selective agent (IV).

Put the Following Steps of the Production of a Recombinant