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In the Biosynthetic Pathway for Conversion from Homoserine to Methionine

Question 25

Multiple Choice

In the biosynthetic pathway for conversion from homoserine to methionine, you identify a Neurospora crassa double mutant Met1/Met2. This mutant will grow only if which supplement(s) are added to the minimal media?
 Growth Medium  Mutant strain  Minimal medium Minimal+cysteine  Minimal+cystathionine Minimal+ homocysteine  Minimal+ methionine Compound accumulating in mutant Controlprototroph +++++None  Met 1+ Homocysteine  Met 2++ Cystathionine  Met 3+++ Cysteine  Met 4++++ Homoserine \begin{array} { l } \text { Growth Medium }\\\hline \begin{array} { l } \text { Mutant }\\ \text {strain }\\\end{array}&\begin{array} { l } \text { Minimal}\\ \text { medium}\\\end{array}&\begin{array} { l } \text { Minimal+}\\ \text {cysteine }\\\end{array}&\begin{array} { l } \text { Minimal+}\\ \text {cystathionine }\\\end{array}&\begin{array} { l } \text {Minimal+ }\\ \text {homocysteine }\\\end{array}&\begin{array} { l } \text { Minimal+}\\ \text { methionine}\\\end{array}&\begin{array} { l } \text { Compound}\\ \text { accumulating}\\ \text { in mutant}\\\end{array}\\\hline\begin{array} { l } \text { Control}\\ \text {prototroph }\\\end{array}&+&+&+&+&+& \text {None }\\\text { Met } 1&-&-&-&-&+&\text { Homocysteine }\\\text { Met } 2&-&-&-&+&+&\text { Cystathionine }\\\text { Met } 3&-&-&+&+&+&\text { Cysteine }\\\text { Met } 4&-&+&+&+&+&\text { Homoserine }\\\end{array}
 In the biosynthetic pathway for conversion from homoserine to methionine, you identify a Neurospora crassa double mutant Met1/Met2. This mutant will grow only if which supplement(s)  are added to the minimal media?   \begin{array} { l  } \text { Growth Medium }\\ \hline \begin{array} { l  }   \text { Mutant }\\ \text {strain }\\ \end{array}&\begin{array} { l  }   \text { Minimal}\\ \text { medium}\\ \end{array}&\begin{array} { l  }   \text { Minimal+}\\ \text {cysteine }\\ \end{array}&\begin{array} { l  }   \text { Minimal+}\\ \text {cystathionine }\\ \end{array}&\begin{array} { l  }   \text {Minimal+ }\\ \text {homocysteine }\\ \end{array}&\begin{array} { l  }   \text { Minimal+}\\ \text { methionine}\\ \end{array}&\begin{array} { l  }   \text { Compound}\\ \text {  accumulating}\\ \text {  in mutant}\\ \end{array}\\ \hline \begin{array} { l  }   \text { Control}\\ \text {prototroph }\\ \end{array}&+&+&+&+&+& \text {None }\\ \text { Met } 1&-&-&-&-&+&\text { Homocysteine }\\ \text { Met } 2&-&-&-&+&+&\text { Cystathionine }\\ \text { Met } 3&-&-&+&+&+&\text { Cysteine }\\ \text { Met } 4&-&+&+&+&+&\text { Homoserine }\\ \end{array}      A)  cystathionine and homocysteine B)  homocysteine only C)  cysteine only D)  methionine only E)  homoserine only


A) cystathionine and homocysteine
B) homocysteine only
C) cysteine only
D) methionine only
E) homoserine only

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