There is a hypothetical gene in mice that produces a substance that induces twitchiness in hind leg muscles. A female mouse of a true- breeding twitchy strain is mated with a male of a true- breeding non- twitchy strain. All progeny are twitchy. Twitchiness may simply be a dominant trait caused by a nuclear allele, or it could be due to mtDNA. Which of these procedures would you use to provide evidence for your hypothesis that twitchiness is due to mtDNA?
A) Sequence the mtDNA of the twitchy male offspring.
B) Backcross twitchy female offspring with normal fathers.
C) Breed twitchy male progeny with normal females to show no paternal inheritance.
D) Breed twitchy female with twitchy male F1 mice to verify there is maternal inheritance.
E) Sequence the mtDNA of the twitchy mother.

Question

Quizplus · Accepted Answer

C) Breeding twitchy male progeny with normal females to show no paternal inheritance would provide evidence for mtDNA involvement, as mtDNA is maternally inherited and would not be passed on by males.

There Is a Hypothetical Gene in Mice That Produces a Substance