Which of the following lists the steps for cDNA library preparation in the correct order? I. Partially degrade the mRNA with RNase H
II. Isolate mRNA from a specific cell/ tissue with an oligo- dT primer that hybridizes to poly- A tail
III. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV. Ligate cDNAs into a cloning vector
V. Create a single- stranded DNA molecule complementary to the mRNA using reverse transcriptase
A) II, V, IV, III, I
B) II, V, I, III, IV
C) I, II, III, IV, V
D) II, III, V, I, IV
E) V, III, I, IV, II
Correct Answer:
Verified
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