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The Electrophoresis Gel Shown Below Is from a DNase I

Question 3

Multiple Choice

The electrophoresis gel shown below is from a DNase I footprint analysis of an operon transcription control region. Separate samples of control- region DNA were exposed to DNase I and run in the following lanes: uprotected DNA is in lane 1, DNA protected by repressor is in lane 2, and DNA protected by RNA polymerase is in lane 3. The electrophoresis gel shown below is from a DNase I footprint analysis of an operon transcription control region. Separate samples of control- region DNA were exposed to DNase I and run in the following lanes: uprotected DNA is in lane 1, DNA protected by repressor is in lane 2, and DNA protected by RNA polymerase is in lane 3.   Based on the gel, what nucleotide positions are occupied by the repressor and RNA polymerase? A)  19 through 29 by the repressor and 7 through 24 RNA polymerase B)  1 through 18 by the repressor and 1 through 6 by RNA polymerase C)  30 through 35 by the repressor and 7 through 24 by RNA polymerase D)  1 through 18 by the repressor and 25 through 35 by RNA polymerase E)  19 through 29 by the repressor and 1 through 6 by RNA polymerase Based on the gel, what nucleotide positions are occupied by the repressor and RNA polymerase?


A) 19 through 29 by the repressor and 7 through 24 RNA polymerase
B) 1 through 18 by the repressor and 1 through 6 by RNA polymerase
C) 30 through 35 by the repressor and 7 through 24 by RNA polymerase
D) 1 through 18 by the repressor and 25 through 35 by RNA polymerase
E) 19 through 29 by the repressor and 1 through 6 by RNA polymerase

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