Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured (usually by soaking the gel in NaOH). Why is this step necessary?

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The Answer of Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured (usually by soaking the gel in NaOH). Why is this step necessary?

Before Transferring DNA from a Gel to the Membrane in Southern