In a mutant E. coli strain, there was a mutation in the malate dehydrogenase gene that resulted in a stop codon at amino acid position #14. From subcultures of this strain, a colony was obtained that was capable of making malate dehydrogenase. Your approach to examining this phenomenon should be to examine
A)the DNA and plasmids for the incorporation of a second complete copy of the malate dehydrogenase gene.
B)the DNA sequence to see if a suppresor mutation has occurred.
C)other proteins to see if other stop codons are ignored, indicating the presence of a suppresor tRNA.
D)the DNA sequence to see if a reversion has occurred.
E)all of the above

Question

Quizplus · Accepted Answer

All options are valid approaches to investigate how the colony regained the ability to produce malate dehydrogenase, including checking for a second gene copy, suppressor mutations, suppressor tRNA, or a reversion mutation.

In a Mutant E