In the Process of Isolating DNA for Transcription and Translation

In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and ligate the gene into a plasmid vector. However, even though all of the controls work, no transcription is obtained using your fragment. Working backward, you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene. It is possible that during restriction digestion

A) the promoter was removed.
B) the RNA polymerase became inactivated.
C) the termination signal was removed.
D) primase could not bind.
E) none of the above