The Normal Gene for Hemoglobin Was Isolated, Cloned, and Subjected

The normal gene for hemoglobin was isolated, cloned, and subjected to restriction enzyme digestion with BamHI. Following gel electrophoresis, three bands are routinely obtained. However, following the same procedure with the hemoglobin gene from an individual with sickle cell anemia, four bands are routinely obtained. These data suggest that

A) the assay was not performed correctly.
B) the mutation associated with sickle cell anemia results in DNA that breaks easily.
C) the mutation associated with sickle cell anemia results in the loss of a BamHI cut site.
D) the mutation associated with sickle cell anemia results in several new enzyme cut sites for a variety of restriction enzymes.
E) the mutation associated with sickle cell anemia results in the gain of a BamHI cut site.