In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because
A)the dideoxynucleotide analogs have no 2'- OH group to continue DNA synthesis.
B)the dideoxynucleotide analogs have no 3'- OH group to continue DNA synthesis.
C)the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
D)the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
E)the dideoxynucleotide analogs have no 5'- OH group to continue DNA synthesis.

Question

Quizplus · Accepted Answer

The dideoxynucleotide analogs lack a 3'-OH group, which is necessary for forming the phosphodiester bond with the next nucleotide, thus terminating DNA synthesis.

In the Sanger Method of DNA Sequencing, Incorporation of the Nucleotides