In a Lysosomal Storage Disease, the Hydrolases of Lysosomes Are

In a lysosomal storage disease, the hydrolases of lysosomes are released from the cells, rather than being isolated into vesicles. What is one possible mechanism for this result?

A) A proton gradient within vesicles was not generated.
B) There is a mutation in all hydrolase genes that results in loss of the signal peptide.
C) Lysosomal enzymes were not phosphorylated in the rER.
D) There is a lack of mannose- 6- phosphatase activity.
E) ER was unable to produce lysosomal vesicles.