Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following gene combinations would identify those mouse cells that were the result of homologous recombination?
A) NeoR only
B) Tk only
C) NeoR and Tk
D) Neither NeoR or Tk

Question

Quizplus · Accepted Answer

The NeoR gene confers resistance to neomycin, allowing for selection of cells where homologous recombination has occurred. The Tk gene is used to select against non-homologous recombination, as cells with Tk will be sensitive to ganciclovir. Therefore, cells with only NeoR indicate successful homologous recombination.

Using the NeoR/Tk System of Introducing Gene Replacements into Mice