DNA ligase is needed in a cloning experiment for what reason?
A) To proofread the vector for mistakes
B) To reestablish the stable sugar-phosphate backbone of the DNA molecule
C) To allow the vector to enter into the cell
D) As a selectable marker

Question

Quizplus · Accepted Answer

DNA ligase is an enzyme that joins the sugar-phosphate backbones of DNA molecules. In a cloning experiment, DNA ligase is used to join the ends of the vector DNA molecule to the ends of the DNA fragment that is being cloned. This creates a continuous DNA molecule that can be replicated by the host cell.

DNA Ligase Is Needed in a Cloning Experiment for What