___________ conditions are essential in a Southern blot experiment if the researcher wants to identify the closest DNA match to the probe.
A)High stringency
B)Low stringency

Question

Quizplus · Accepted Answer

High stringency conditions allow for hybridization of only DNA fragments that have a high degree of complementarity with the probe. This ensures that only the closest DNA match to the probe is identified, which is essential for the success of a Southern blot experiment. Low stringency conditions, on the other hand, allow for hybridization of DNA fragments that have only partial complementarity with the probe, resulting in a high background signal and reduced specificity.

___________ Conditions Are Essential in a Southern Blot Experiment If