What is the purpose of SDS in SDS-PAGE?
A) to selectively bind the target protein
B) to maintain buffer pH in the gel
C) to cause the separation to be on the basis of molecular weight only
D) to initiate polymerization of acrylamide to form a gel

Question

Quizplus · Accepted Answer

SDS (sodium dodecyl sulfate) is a detergent that denatures proteins and imparts a uniform negative charge, allowing them to be separated based on molecular weight during electrophoresis.

What Is the Purpose of SDS in SDS-PAGE