Protein folding can be followed in the laboratory by measuring
A)viscosity.
B)electrophoretic mobility and gel filtration.
C)UV absorption and light rotation.
D)A,B,and C above.
E)A and C above.

Question

Quizplus · Accepted Answer

Protein folding can be followed in the laboratory by measuring UV absorption and light rotation (Option C) to detect changes in the secondary structure of the protein, and viscosity (Option A) can be used to detect changes in the tertiary structure. Electrophoretic mobility and gel filtration (Option B) are not direct methods to measure protein folding but can be used to detect changes in protein size or conformation after folding. Therefore, only Options A and C together provide the best choice for measuring protein folding.

Protein Folding Can Be Followed in the Laboratory by Measuring