The assumptions made in calculating the Michaelis-Menten Equation include A) that the formation and decomposition of ES is the same for a period of time. B) that the concentration of the substrate is much greater than the concentration of E. C) that the value of k_-₂can be ignored. D) A, B and C E) A and B only

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The Michaelis-Menten equation assumes that the formation and decomposition of the enzyme-substrate complex (ES) reach a steady state (A), the substrate concentration is much greater than the enzyme concentration (B), and the reverse reaction from product to ES (k-2) is negligible (C).

The Assumptions Made in Calculating the Michaelis-Menten Equation Include