The K_mvalues for enzyme reactions such as A + B → C + D A) cannot be determined using the Lineweaver-Burk plot analysis. B) can be determined by holding one A or B) at high concentration, while varying the concentration of the other substrate). C) can be determined for one substrate and not the other. D) do not indicate the efficiency of the enzyme.

Question

Quizplus · Accepted Answer

The K_m value can be determined by holding one substrate at a high concentration while varying the concentration of the other substrate. This is because the K_m value is the concentration of substrate at which the reaction rate is half-maximal.

The Km values for Enzyme Reactions Such as a

The K_mvalues for Enzyme Reactions Such as a