Which statement is true about the removal of reversible inhibitors from enzyme solutions?
A)PAGE is the primary technique used for this purpose.
B)Dialysis and gel filtration are often used.
C)These inhibitors cannot be separated from the enzyme without treatment with diisopropylfluorophosphate.
D)Removal of reversible inhibitors is extremely difficult and can be achieved only after many purification steps.

Question

Quizplus · Accepted Answer

Dialysis and gel filtration are commonly used for the removal of reversible inhibitors from enzyme solutions. PAGE is not typically used for this purpose, and diisopropylfluorophosphate is not necessary for removal of reversible inhibitors. The difficulty of removal can vary depending on the specific inhibitor and enzyme, but it is not generally considered to be extremely difficult.

Which Statement Is True About the Removal of Reversible Inhibitors