The following pH dependence was found for the activity of a certain enzyme-catalyzed reaction.
If it is known that the only two ionizable residues in the active site are both glutamates, which conclusion can be drawn?
A) The glutamates have different microenvironments which cause their pKaʹs to differ.
B) One of the glutamates must be amidated.
C) Both glutamates have a pKa equal to 5.0.
D) Both glutamates are deprotonated during the reaction.
Correct Answer:
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