Experiments on the bacterial serine protease subtilisin show that even when all three residues of the catalytic triad are mutated, the catalytic rate of the enzyme is still 3000 times the uncatalyzed reaction rate. Which mode of catalysis is likely responsible for this remaining catalytic activity?

Question

Quizplus · Accepted Answer

Transition-state stabilization is likely responsible for the remaining catalytic activity, as it can still occur even when the catalytic triad is mutated, by stabilizing the transition state of the substrate, thus lowering the activation energy.

Experiments on the Bacterial Serine Protease Subtilisin Show That Even