Many dehydrogenases can be assayed by measuring the decrease of absorption of NAD⁺or the increase of absorption of NADH.

Question

Quizplus · Accepted Answer

Many dehydrogenases catalyze the transfer of hydrogen from a substrate to NAD⁺, resulting in the reduction of NAD⁺ to NADH. The decrease in absorption of NAD⁺ at 340 nm and the increase in absorption of NADH at 340 nm can be used to monitor the progress of the reaction and quantify the activity of the dehydrogenase.

Many Dehydrogenases Can Be Assayed by Measuring the Decrease of Absorption