Mechanical shearing is often the preferred method of fragmenting genomic DNA when constructing a genomic DNA library. Why would this method be preferred over complete digestion with restriction enzymes?
A) Enzymes might produce fragments that are too small or too large to be cloned.
B) Enzyme cleavage can interrupt important gene sequences, in which case not all genes may be represented in the library.
C) Shearing ensures the library contains overlapping fragments of DNA, which makes it possible to perform a chromosome walk.
D) All of the above

Question

Quizplus · Accepted Answer

Mechanical shearing is preferred because it avoids the issues of restriction enzymes producing fragments of unsuitable sizes, potentially interrupting important gene sequences, and ensures overlapping fragments for comprehensive library representation.

Mechanical Shearing Is Often the Preferred Method of Fragmenting Genomic