To perform the coagulase test on a strain of bacteria that is believed to be S. aureus, the ﬁrst step is to inoculate a tube of ______ with the strain of interest.

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The coagulase test involves inoculating a tube of blood plasma with the strain of interest because the coagulase enzyme, produced by some Staphylococcus aureus strains, coagulates the fibrinogen in plasma, leading to clot formation. This test helps differentiate S. aureus, which is coagulase-positive, from other Staphylococcus species that are coagulase-negative.

To Perform the Coagulase Test on a Strain of Bacteria