You Have Inoculated a Clostridium Species into a Tube of Media

You have inoculated a Clostridium species into a tube of media. After incubation, you observe that there is no growth, and you remember that Clostridium is an obligate anaerobe. What is the best explanation for the observed lack of growth?

A) There is not enough water in the environment for Clostridium to grow.
B) Oxygen in the medium destroys the enzymes needed by Clostridium to undergo aerobic respiration.
C) Clostridium does not have enzymes to process toxic oxygen.
D) There is not enough oxygen for use when toxic oxygen is present.