Which of the following is NOT a recent discovery that has had a huge impact on the understanding of microbiology?

Question

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All the options listed (restriction enzymes, PCR technique, human microbiome project, and small RNAs) are significant discoveries that have greatly impacted the field of microbiology. Restriction enzymes have revolutionized genetic engineering, PCR has vastly improved the ability to detect and amplify DNA sequences, the Human Microbiome Project has expanded our understanding of the role of microorganisms in human health and disease, and the discovery of small RNAs has unveiled new mechanisms of gene regulation.

Which of the Following Is NOT a Recent Discovery That