Two fluorescent proteins (protein 1 and protein 2)are used in a FRET experiment and are illuminated with light that excites protein 1. If the proteins are close enough for energy transfer to occur, what wavelength(s)of light will be detected?

Question

Quizplus · Accepted Answer

If the proteins are close enough for energy transfer to occur, the excitation energy from protein 1 will be transferred to protein 2, leading to emission of light by protein 2 at a different wavelength. Therefore, the wavelength of light that will be detected is the light that is emitted by protein 2 (choice D).

Two Fluorescent Proteins (Protein 1 and Protein 2)are Used in a FRET